Holmes,-W-M (Division of Clinical Neuroscience, University of Glasgow, Glasgow (United Kingdom)); Maclellan,-S (The Doctoral Training Centre, Bioengineering Unit, University of Strathclyde (United Kingdom)); Condon,-B (Division of Clinical Neuroscience, University of Glasgow, Glasgow (United Kingdom)); Dufes,-C (Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow (United Kingdom)); Evans,-T-R-J (Centre for Oncology and Applied Pharmacology, Beatson Laboratories, University of Glasgow, Glasgow (United Kingdom)); Uchegbu,-I-F (The Doctoral Training Centre, Bioengineering Unit, University of Strathclyde (United Kingdom)); Schaetzlein,-A-G (The Doctoral Training Centre, Bioengineering Unit, University of Strathclyde (United Kingdom) The investigation of mouse flank tumours by magnetic resonance imaging (MRI) is limited by the achievable spatial resolution, which is generally limited by the critical problem of signal-to-noise ratio. Sensitivity was improved by using an optimized solenoid RF micro-coil, built into the animal cradle. This simple design did not require extensive RF engineering expertise to construct, yet allowed high-resolution 3D isotropic imaging at 60 x 60 x 60 mu m sup 3 for a flank tumour in vivo, revealing the heterogeneous internal structure of the tumour. It also allowed dynamic contrast enhanced (DCE) experiments and angiography (MRA) to be performed at 100 x 100 x 100 mu m sup 3 resolution. The DCE experiments provided an excellent example of the diffusive spreading of contrast agent into less vascularized tumour tissue. This work is the first step in using high-resolution 3D isotropic MR to study transport in mouse flank tumours.
